Multiphasic chip performance characterization using human thyroid cancer cell line SW1736 and human plasma
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https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=a4c480cd61ed4f91adc58f2185027f64
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资源简介:
Development of an online peptide fractionation system comprising a multiphasic LC chip that integrates reversed phase and strong cation exchange chromatography upstream of the MS based on the multi-dimensional protein identification technology (mudPIT). Chips were assessed in terms of chromatographic as well as protein and peptide identification reproducibility using five-step fractionation of 0, 2, 50, 500 and 1500 mM ammonium acetate. Further, a comparison of multiphase LC chip protein and peptide identification versus conventional reversed phase LC-MSMS was performed.
创建时间:
2017-09-20



