Growth Defects in <i>slik</i> Mutants

(A) Heterozygous slik1/+ control and homozygous slik1 mutant larvae after 5 d of growth under uncrowded conditions. (B) Growth and survival characteristics of slik1/+ control and homozygous slik1 mutant larvae. The percentage of animals at each developmental stage on the indicated days are shown by the colored bars. (Left) slik1/+ control animals. (Middle) Homozygous slik1 mutant animals. (Right) Percent expected homozygous slik1 mutant females rescued by expression of Slik under control of armadilloGAL4. Note that only females received both the armadilloGAL4 driver and the UAS-slik transgene. In competition with rescued females, male larvae die earlier than when all animals are homozygous mutant. The few surviving males are included in the107% recovery at 5 d. (C) Comparison of slik1/+ control females (left) with homozygous slik1 mutant females rescued by expression of Slik under control of armadilloGAL4. Note the reduced body size of the rescued flies. (D–F) Larval internal organs labeled by BrdU incorporation. (D and E) Control larvae fed BrdU from 76 to 92 h and 56 to 72 h AEL, respectively. All nuclei are brown, indicating BrdU incorporation during endoreplication. (F) slik1 mutant larva fed from 76 to 92 h. Few nuclei were labeled. Note that the size is comparable to the much younger wild-type control.