Stability of ΔψM during culturing of human primary β-cells in whole islet and dispersed cell cultures.

(A) ΔψM in 3 mM glucose (rich medium) as a function of days spent in whole-islet suspension culture before dispersion. ΔψM was measured 3–4 days after dispersing islet cells. (B) ΔψM hyperpolarization evoked by 16 mM glucose corresponding to (A). (C) ΔψM in 3 mM glucose (rich medium) as a function of days spent in dispersed islet cell culture after an arbitrary length of whole islet culturing. (D) ΔψM hyperpolarization evoked by 16 mM glucose corresponding to (C).