Transcriptomic analysis of mouse embryonic stem cells and differentiated cells upon OGT inhibition. Transcriptomic analysis of mouse embryonic stem cells and differentiated cells upon OGT inhibition

To ask whether perturbations on global O-GlcNAc levels could alter embryonic stem cell identity and differentiation processes, we explored the gene expression of mouse embryonic stem cells (mESCs) and retinoic acid (RA)-inducted differentiated cells with or without OGT inhibition by per-acetylated 2-acetamido-2-deoxy-5-thio-D-glucopyranose (Ac45SGlcNAc, 5S). Overall design: We performed gene expression profiling analysis using data obtained from RNA-seq of mESCs and retinoic acid (RA)-inducted differentiated cells with or without OGT inhibition by Ac45SGlcNAc. For OGT inhibition, mESCs were treated with 25 μM Ac45SGlcNAc for 48 h. For inducing mESC differentiation, the cells pre-treated with DMSO or Ac45SGlcNAc were then incubated with 1 μM RA for 48 h. N_NC: normal mouse embryonic stem cells, N_5S: 5S treated mouse embryonic stem cells, DIF_NC: normal differentiated cells, DIF_5S: 5S treated differentiated cells.