A framework for gene mapping in wheat demonstrated using the Yr7 yellow rust resistance gene

We mapped the yellow rust resistance gene Yr7 to a low-recombination region of chromosome 2B in wheat. We used mapping-by-sequencing of resistant and susceptible DH bulks to identify a candidate gene in the interval showing high homology to a previously suggested Yr7 candidate and to populate the Yr7 interval with a higher density of polymorphisms. Our mapping-by-sequencing methodology is applicable to any trait and our results validate the approach in wheat, where with a near complete reference genome sequence, we are able to define a small interval containing the causative gene.