PRL-3 induces Kupffer cells to recruit MDSCs in liver through exosome ITGavß5

Previous studies have found that PRL-3 promotes liver metastasis of colon cancer, but whether it is involved in promoting liver PMN formation remains unclear.It was found that MDSC is the main cellular component of PMN formation and plays an immunosuppressive role.We found that PRL-3 can up-regulate the expression of ITGavß5 in exosomes of colon cancer cells through proteomics identification and extractor identification, and further verified that exosome ITGavß5 uptake by Kupffer cells can participate in inducing liver infiltration of MDSCs by exosome pre-education and mouse liver metastasis model construction,thus promoting liver metastasis of colon cancer. Then we extracted exosomes from colon cancer cells, induced Kupffer cells in vitro, and conducted RNA-seq sequencing to screen and identify genes involved in regulating MDSC infiltration. Overall design: We carefully selected Kupffer cells with robust viability and exposed them to MC38-PRL-3 Exo and MC38-VT Exo for 48 hours (with biological replicates, n=2/group). Total RNA was extracted from Kupffer cells using the Trizol method. The mRNA library construction, sequencing, and bioinformatics analysis of the sequencing results were outsourced to IGE Biotechnology