Fluorescence and CD spectra of MSL1.

Fluorescence (A) and far-UV CD (B) spectra for MSL1 in 50 mM acetic/acetate buffer (pH 4.5). Conditions for both experiments were 20 µM of protein and 25°C. Thermal denaturation of MSL1 followed by fluorescence (C) and far-UV CD (D). Fluorescence lines represent the thermal denaturations of MSL1 at an emission wavelength of 315 nm, either at an excitation wavelength of 278 nm (blue line) or 295 nm (red line). Similar results were obtained when emission wavelength was monitored at 330 nm or 350 nm. Spectra were acquired in either 1 cm- (fluorescence) or 0.1 cm- (far-UV CD) path-length cells.