SATAY sequencing data from multiple biological replicates of a wild-type strain and a bem1?bem3?nrp1? mutant strain

Functional defects resulting from deleterious mutations can often be restored during evolution by compensatory mutations elsewhere in the genome. Importantly, this process can generate the genetic diversity seen in networks regulating the same biological function in different species. How the options for compensatory evolution depend on the molecular interactions underlying these functions is currently unclear. This dataset, comprising multiple SATAY transposon mutagenesis libraries, was used to examine how gene deletions that compensate for defects in the polarity pathway of Saccharomyces cerevisiae impact the fitness landscape on a genome-wide scale. In total, 12 SATAY libraries were created, consisting of 6 biological replicate experiments of a wild-type strain (yEK19) and 6 biological replicates of a bem1?bem3?nrp1? mutant (yEK23). For each strain, the letter following the strain name (for example, yEK19a and yEK23a) represents different colonies taken from the same transformation plate during strain construction. Following strain construction, the different replicates of yEK19 and yEK23 were transformed with plasmid pBK549 and multiple colonies were selected from the transformation plate to generate the SATAY libraries. The numbers at the end of each strain name (for example, yEK19a_6 and yEK23a_23) indicate different colonies that were picked from the transformation after the transformation with plasmid pBK549.