Effects of different concentrations of degradation products of magnesium and magnesium alloys on osteogenic precursor cells

Magnesium-based alloys have a great potential to become orthopedic degradable materials for internal fixation implants, and magnesium-based implants have been shown to influence the surrounding bone structure. To understand the effects of magnesium-based implants on bone healing and remodeling, we investigated the effects of the extracts of pure magnesium and three magnesium alloys on the proliferation and differentiation of osteogenic precursor cells in vitro. MC3T3-E1 cells were selected as the study objects. Cell culture medium was used to extract the aforementioned metal materials, and the degradation products and extracts were subjected to a compositional analysis. Subsequently, the extracts were diluted to different concentrations for cell proliferation/differentiation cultures. The MC3T3-E1 proliferation and differentiation were evaluated based on the cell viability, alkaline phosphatase activity, total protein content, and cellular ability to form calcified nodules in the extrac...