Effects of different concentrations of degradation products of magnesium and magnesium alloys on osteogenic precursor cells

Magnesium-based alloys have a great potential to become orthopedic degradable materials for internal fixation implants, and magnesium-based implants have been shown to influence the surrounding bone structure. To understand the effects of magnesium-based implants on bone healing and remodeling, we investigated the effects of the extracts of pure magnesium and three magnesium alloys on the proliferation and differentiation of osteogenic precursor cells in vitro. MC3T3-E1 cells were selected as the study objects. Cell culture medium was used to extract the aforementioned metal materials, and the degradation products and extracts were subjected to a compositional analysis. Subsequently, the extracts were diluted to different concentrations for cell proliferation/differentiation cultures. The MC3T3-E1 proliferation and differentiation were evaluated based on the cell viability, alkaline phosphatase activity, total protein content, and cellular ability to form calcified nodules in the extrac...

镁基合金在作为骨科内固定植入用可降解材料方面具有巨大应用潜力，且已有研究证实镁基植入物可对周围骨组织结构产生影响。为阐明镁基植入物对骨愈合与骨重塑的影响，本研究探讨了纯镁及三种镁合金浸提液对体外成骨前体细胞（osteogenic precursor cells）增殖与分化的作用。本研究选用MC3T3-E1细胞作为实验对象，采用细胞培养液对上述金属材料进行浸提，并对其降解产物及浸提液开展成分分析；随后将浸提液稀释至不同浓度，用于细胞增殖与分化培养。本研究通过细胞活力、碱性磷酸酶（alkaline phosphatase）活性、总蛋白含量以及细胞钙化结节形成能力，对MC3T3-E1细胞的增殖与分化水平进行评估。（原文后续内容截断为extrac...）