Foxa1 mutations co-opt nascent transcription factor networks in partnership with androgen receptor to enhance prostate tumorigenicity

Foxa1 mutants induce distinct transcriptomic and DNA accessibilities, prominent aberrant epithelial lineage gene expression, and enhance prostate tumorigenicity in vivo Overall design: Murine organoids established from adult mouse prostate were transduced with lentivirus for pCW-EV and pCW-Foxa1 (WT and mutant cDNA). Following sorting and 3D culturing organoids, single nuclei were extracted from mouse prostate organoids. Joint scRNA- and scATAC libraries were prepared using the 10x Genomics platform.