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To study the phylogenetic relationship of H.dentata, a phylogenetic analysis was performed based on the complete chloroplast genome sequences of 16 species including the H.dentata chloroplast genome assembled in this study from Orchidaceae and two species from other family. The chloroplast genomes of 17 species were downloaded from the NCBI GenBank database. The sequences were aligned using MAFFT v7 (Mayor et al. 2000). Then we constructed the phylogenetic tree using Maximum Likelihood (ML) method by RAxML 7.0.4 (Stamatakis 2006) with 1000 replicates under the GTR + CAT model. Iris setosa and Iris uniflora were designated as outgroup species. The phylogenetic tree indicated that H.dentata was most closely related to H.chejuensis, as expected (Figure 1). The data of the complete cp genome of H.dentata provided an essential clue for better understanding the phylogeney and biodiversity of Habenaria.