Role of different variants of leptin receptor in human adrenal tumor types

Quantitative PCR (qPCR) results showing mRNA expression levels of leptin receptor isoforms (LepR1–LepR5) in adrenal tumor and control tissue samples. RNA was isolated from adrenal tissues, reverse-transcribed to cDNA, and analyzed using SYBR Green qPCR on a Bio-Rad CFX96 system. B2M served as the reference gene. Each column represents: B2M.Cq – reference gene Cq; lep1.Cq, lep2.Cq, lep3.Cq, lep5.Cq – Cq values for leptin receptor transcript variants; lep1_dd–lep5_dd – ΔCq (difference between target and B2M); ddCt_lep1–ddCt_lep5 – ΔΔCq relative to control mean; and 2^-ddCt_lep1–2^-ddCt_lep5 – normalized relative expression (fold change). Empty cells indicate undetectable expression. Primer pairs used in the qPCR correspond to specific leptin receptor transcript variants: lep1 → transcript variant 1 (LepR1), lep2 → variants 2 and 4 (LepR2&LepR4), lep3 → variants 3 and 6 (LepR3&LepR6), lep5 → variant 5 (LepR5). Cq values represent the number of amplification cycles required to reach the fluorescence threshold and are inversely related to expression level. Relative expression was calculated using the 2^-ΔΔCq method.