Human Platelet Lysate-Cultured Adipose-Derived Stem Cell Sheets Promote Angiogenesis and Accelerate Wound Healing via CCL5 Modulation. Human Platelet Lysate-Cultured Adipose-Derived Stem Cell Sheets Promote Angiogenesis and Accelerate Wound Healing via CCL5 Modulation

To further differentiate the gene expression of HPL-cultured or FBS-cultured ASC sheets, we have employed microarray analysis. ASCs were isolated from the subcutaneous fat tissue of four non-diabetic, non-smoking female donors with an average age of 45 y (32–57 y) and an average body mass index of 24.6 (21.0–26.6). Significantly up-regulated or down-regulated genes are highlighted in the HPL-cultured ASC sheets. Pathway enrichment analysis showcasing the top 10 hallmark gene-sets from MSigDB significantly enriched in up-regulated (top) or down-regulated (bottom) genes. The heatmap represented the expression patterns of angiogenesis-related genes between the HPL-cultured and FBS-cultured ASC sheets. Overall design: Adipose-derived stem cells (ASCs) were cultured with 5%HPL or 10% FBS for 7 day-sheet fabrication. Angiogenesis-related genes induced in HPL-cultured ASC sheets or the FBS counterpart were investigated.