A Comparative Transcriptomics Analysis Reveals Ethylene Glycol Derivatives of Squalene Ameliorate Excessive Lipogenesis and Inflammatory Response in 3T3-L1 preadipocytes cells

[Note: For black soybean's neuroinflammation study, please refer to accession number: GSE270419] Transcriptomic analysis reveals the anti-excessive lipogenesis and anti-inflammatory effects of SQ and its derivatives on preadipocyte differentiation We horizontally compared the effects of SQ and its four derivatives on preadipocyte differentiation and anti-inflammation. Di- and tetra-SQ can significantly improve the lipid metabolism function and anti-inflammatory effect of adipocytes, and ameliorate excessive production. RNA was extracted using Isogen (Nippon Gene Co. Ltd., Toyama, Japan). The integrity of RNA was quantified using NanoDrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). Clariom S assay system (Thermo Fisher, Japan) was used on duplicate RNA samples of each group. In accordance with the user manual, complementary DNA (cDNA) for microarray was generated using the GeneChip WT Plus Reagent kit (Thermo Fisher Science, Japan). Samples were hybridized using Clariom S GeneChip microarray kit (Thermo Fisher Science, Japan). After washing and staining, images were captured, and then CEL data were generated using the GeneChip Scanner 3000 (Thermo Fisher Science, Japan). The raw CEL data were normalized using the transcriptome analysis console (TAC) software ver.4.0.1 following the SST-robust multichip average (SST-RMA) algorithm (Thermo Fisher Scientific, Japan).