Spiroplasma prevalence in D. hydei following passage under four different thermal regimes

The data represents screening results of F3 flies of D. hydei for Spiroplasma following two generations of passage at different temperatures. Column A: Origin of fly/Spiroplasma isoline. Two levels: Mexico or Cambridge isolines. Column B: Temperature. Temperature during passage over previous two generations. Four levels: 25 °C, 18°C, 18/15°C (diurnal variation), 15 °C. Column C: N of flies tested for Spiroplasma, which had passed QC for DNA template amplifiability using COI prmers. Column D: N of flies scoring as infected on Spiroplasma PCR assay. Column E: Prevalence. Point estimate of proportion flies infected (Column D/Column C) Column F: Upper confidence interval for Spiroplasma prevalence, calculated through binomial sampling. Column G: Lower confidence interval for Spiroplasma prevalence, calculated through binomial sampling.