Functional redundant activities between Nkx2.5 and Nkx2.7 in zebrafish heart development.

For rescue study, the Nkx2.5 mRNA was co-injected with Nkx2.7-MO into embryos derived from zebrafish transgenic line Tg(cmlc2::GFP). The percentages of heart defects were decreased compared to phenotypes which occurred in the embryos injected with Nkx2.7-MO alone. Similarly, either the Nkx2.5 or Nkx2.7 mRNA enabled embryos to be rescued from the defects as a result of the injection of Nkx2.5/2.7-MO. n: total number of embryos analyzed.