RNA-Seq of susceptible and resistant melon (Cucumis melo L.)

Cucumis Melo cultivars TG1 (powdery mildew resistant) and TG5 (powdery mildew susceptible) were used for inoculation with powdery mildew. TG1 and TG5 were grown in a greenhouse. At the 3-5 leaves stage and when plants were approximately 15-20 cm high, 3 plants of each cultivar were inoculated with powdery mildew by cutting the leaf blades at mid-length with sterile scissors previously dipped in the spore suspension. Three plants of each cultivar were mock-inoculated with sterile liquid medium to use as negative controls. Leaf samples distal from the wound site were taken before infection (0 days, control) and after 1, 2, 3 and 5 days, respectively. Three biological replications were prepared at each time point.