Analysis of complementation of LJ14 () harboring pTrc-RRF (sectors 1), pBR- (sectors 2) and pTrc99C (sectors 3)

<b>Copyright information:</b>Taken from "Evidence for a role of initiation factor 3 in recycling of ribosomal complexes stalled on mRNAs in "Nucleic Acids Research 2005;33(17):5591-5601.Published online 30 Sep 2005PMCID:PMC1240113.� The Author 2005. Published by Oxford University Press. All rights reserved Overnight cultures were streaked on LB agar ampicillin plates, and incubated for 36 h at the indicated temperatures. Immunoblot analysis of IF3 levels in total cell extracts (?15 �g) of LJ14 harboring pTrc-RRF (lane 1), pBR- (lane 2) or vector alone, pTrc99C (lane 3). In lane 4, ?0.2 �g IF3 was analyzed as marker. A Coomassie blue stained gel is shown in the above panel (i) as a control for equal loading of total proteins. Band corresponding to IF3 is indicated in the immunoblot in the lower panel (ii). Analysis of growth of transformants of various derivatives of LJ14 () and STL1670 in LB broth containing ampicillin, at 30�C (left) and 37�C (right). Saturated cultures grown at 30�C were inoculated (0.1%) into LB broth containing ampicillin and the growth was monitored at 595 nm at regular time intervals. Details of various strains are as shown in the panels.