Microarray Expression Analysis of Circular RNAs in Pancreatic Ductal Adenocarcinoma

To investigate the expression profile of circular RNAs (circRNAs) in pancreatic ductal adenocarcinoma(PDAC) and to provide more reliable biomarkers and new potential diagnostic and therapeutic targets for PDAC. The circRNA expression levels were measured in 3 paracancerous tissues and 3 PDAC tissues using Arraystar Human CircRNA Array Analysis. We identified significantly different expression of circRNAs between the two groups and showed distinguishable circRNAs expression patterns in the samples. The levels and patterns of circRNA expression were significantly different between paracancerous tissue and PDAC tissue. This study evaluated circRNAs expression profiles and their ability to serve as reliable biomarkers and new potential diagnostic and therapeutic targets for PDAC. To investigate the exact mechanism of circRNAs in PDAC, total RNA was extracted from PDAC tissue and paired normal adjacent tissues, and next-generation sequencing was performed to explore the expression profile differences between PDAC tissue and paired normal adjacent groups. The results come from three independent experiments.