DNA methylome of human peripheral blood mononuclear cells from the YH Han Chinese individual.

The methylome reported and analyzed here was generated from the same sample of peripheral blood mononuclear cells (PBMCs) from a consented donor (Homo sapiens) whose genome was deciphered in the YH project. YH is an anonymous male Han Chinese individual who has no known genetic diseases, and whose genome also serves as an Asian reference genome.

Nuclear DNA was extracted and subjected to unbiased, whole-genome bisulfite sequencing (BS-seq) using the Illumina Genome Analyzer. In total, 103.5 Gbp of paired-end sequence data were generated. Of these, 70.4 Gbp (68%) were successfully aligned to either strand of the YH genome with an average mismatch rate of 1.3%, resulting in an average sequencing depth of 12.3-fold per DNA strand or a 24.7-fold overall depth. Of the 18,962,679 CpGs present in the unique haploid part (2.21 Gb) of the YH reference genome sequence, approximately 99.86% were covered by at least one unambiguously mapped read of quality score >14 on either strand, and 92.62% were unambiguously covered on both strands.