Time course of VSMC cell death by MV.

?Monocytes were stimulated with LPS for indicated period of time and microvesicles (MV) were isolated. MVs were then subjected to VSMC and analyzed for cell death using Annexin-V assay (Panel 1) and Crystal violet (CV) assay (Panel 2). Caspase-1 activity (Panel 3) and caspase-3 activity (Panel 4) were measured from MVs and VSMCs respectively. Annexin-V and caspase -1 and 3 assays represented here are average of N = 2 experiments. CV assay is representative of N = 4 experiments.