Flexibility of metabolic rate to temperature coincides with diapause strategy

During dormancy, insects operate on a fixed energy budget and suppress metabolic rate to extend the duration that their energy reserves last. Extending energy stores to last an entire winter can pose a significant challenge, as some habitats have winters that last most of the year. There are cases where insects enter dormancy in mid-summer and remain until the following spring. This multi-season dormancy should pose an even more significant energetic challenge, since these insects must conserve energy during winter, as well as the warmest period of summer. We compared metabolic rate-temperature curves of two related species of Pierid butterflies: Pieris napi, which is dormant through winter, and Anthocharis cardamines, which exhibits a multi-season dormancy. This comparison was conducted at several time points under 18°C and 2°C acclimation conditions. We found that A. cardamines can maintain considerable metabolic suppression when acclimated to high temperatures, which is only maintain..., , # Flexibility of metabolic rate to temperature coincides with diapause mode

Dataset DOI: [10.5061/dryad.8pk0p2p0n](10.5061/dryad.8pk0p2p0n)

## Description of the data and file structure

Respiration and mass data of diapausing *Anthocharis cardamines* and *Pieris napi* pupae that are acclimated to cold (2C) or warm (18C) at several time points after pupation.

### Files and variables

#### File: Ac_Pn_analysis.R

**Description:** This is the R script used for the statistical analyses used. Each section corresponds with the matching section in the manuscript. The order of models matches the order that the stats are described in each section.

#### File: Ac_Pn_mass.csv

**Description:** This file contains the mass data of each species through the experiment.

##### Variables

* id: individual identification
* species: species, AC = Anthocharis cardamines, PN = Pieris napi
* treatment60: temperature treatment from days 30-60 after pupation. In degrees centigrade
* treatment90: temperatur...,