Transcriptome analysis elucidates the potential key genes involved in rib defects in bmp6-deficient silver carp (Hypophthalmichthys molitrix)

This study showcases the effective utilization of CRISPR/Cas9 technology for the purpose of disrupting the bmp6 gene in silver carp, leading to the creation of a chimera in the P0 generation. Transcriptome analysis on ribs of P0 chimeras and wild-type was conducted, leading to the identification of 1333 genes exhibiting differential expression, of which 649 were found to be up-regulated and 684 were found to be down-regulated. This study presents novel findings on the impact of bmp6 gene deletion on the rib development of silver carp, while simultaneously investigating the previously unexplored molecular mechanisms underlying rib defects in fish.