sorghum m6Aseq

The Homogeneous and healthy sorghum seed cultivar used in this study was Jtian 3 provided by Agricultural Research Institute in Hebei Province. The experiment was arranged in a factorial design with one salinity levels 150 mM NaCl; and five GA3 concentrations.There was one salinity levels and five GA3 treatments repeated three times for a total of 36 pots for each time. Then these sorghum seeds were soaked with different concentrations of GA3 solution in darkroom for 12h with a temperature of 25 degree Celsius, Then, the seeds were buried in the wet sand containing 1/2 concentration of Hoagland solution for 48h for pre-germination. Seeds with similar bud length were selected and planted in a plastic tray (50 cm long; 30 cm width; 5 cm high) with holes at the bottom. Each pot (5 cm in top diameter and 2.5 cm in bottom diameter) was filled with quartz sand. Three seeds were planted in each pot at 3 cm seeding depth. The data measurement and sample collection were set on the 40th days after treatment, and three were randomly selected for each treatment as replicates. Then randomly selected 0mg/L GA3 control and 50mgL GA3 concentration treatment were sent to the company for sequencing