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AAV-Delivered Engineered Suppressor tRNA Rescues Visual Function in Mice with an Inherited Retinal Disease (ribo-seq) (PRJCA045216)

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NIAID Data Ecosystem2026-05-10 收录
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https://www.ncbi.nlm.nih.gov/sra/DRP016381
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At the age of two weeks, subretinal injections were performed on the mice under a stereomicroscope. Each eye of a mouse was injected with 1 µL of scAAV8.sup-tRNA at the dose of 1x109 GC/eye. 12 weeks post injection, the eyes were enucleated and retina/RPE/choroidal/sclera complex were dissociated and immediately frozen in liquid nitrogen and ground to power in liquid nitrogen with a mortar and pestle, then dissolved in 400µL of lysis buffer.
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2025-12-11
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