Arteriovenous fistula creation results in cardiac dysfunction and remodeling in a uremic pig model

Supplementary Table S1: primer sequences used for qPCR analysesFigure S1: Ventricular volumes and ejection fraction are unchanged 28 and 42 days after percutaneous transluminal angioplasty (PTA). Pigs were assessed via 4D cardiac MRI at 14-, 28-, and 42-days following PTA. (A) ejection fraction changes in the right and left ventricle are shown, right ventricle ejection fraction (RVEF), left ventricle ejection fraction (LVEF) (B) flow changes through the ventricles are shown, right ventricle stroke volume (RVSV), left ventricle end-systolic volume (LVESV). (C) cardiac output is shown as litres per minute. (D) Flow through the superior vena cava plus the descending aorta is shown as ml per cycle. (E) heart rate at time of each MRI acquisition is shown. Day 14 (D14), n=4. Day 28 (D28), n=4, Day 42 (D42), n=3. ns, not significant.Figure S2: Aortic diameter and cross-sectional area are unchanged 28 and 42 days after percutaneous transluminal angioplasty (PTA). Pigs were assessed via 4D cardiac MRI at 14-, 28-, and 42-days following PTA. Cross sectional area of the ascending (A) and descending (C) aorta are shown at each time point. Diameter of the ascending (B) and descending (D) aorta are shown at each time point. ns; not significant. Day 14, D14. Day 28, D28. Day 42, D42.Figure S3: Characteristics of the left ventricle are unchanged. Pigs were assessed 42-days following PTA and compared to control pigs. (A) Fold change expression vs control is shown for collagen type-I (COL1A1). (B) percent positivity for picrosirius red is shown in the whole ventricle along with representative images of the ventricular section used (C and D). ns; not significant.