Bacterial Isolate Microscopy
收藏DataCite Commons2022-07-20 更新2024-07-29 收录
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https://figshare.com/articles/dataset/Bacterial_Isolate_Microscopy/20326650/1
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<em><strong>Epifluorescence microscopy of gut bacterial isolates after EdU-click</strong></em>. Human gut bacterial isolates which underwent EdU-click with or without EdU were stained with Sybr Green I nucleic acid dye and visualized using epifluorescence microscopy. Green = Sybr Green I, staining all bacterial nucleic acid content; Red = Alexa Fluor 647 azide, staining only newly replicated bacterial DNA. <br> All slides were visualized using the Axiovert 3 widefield inverted microscope (Zeiss) equipped with a Zeiss Axiocam 506 monochrome CCD camera (2752 x 2208 pixels, 4.54 um pixels) and Sola SM solid state light engine, using the 100X objective (PLAN-APOCHROMAT, NA=1.4, OIL, Iris) under oil immersion and the FS 24 (FITC) and FS 32 (Cy5) fluorescence cubes. Images were further processed in Fiji (version 1.53q)
提供机构：
figshare
创建时间：
2022-07-20
