Most relevant differences in terms of gene expression in H9c2 undifferentiated myoblasts vs RA-differentiated H9c2 cells.

H9c2 cells were differentiated for 5 days in a low serum concentrated medium daily supplemented with RA. Both groups, undifferentiated and RA-differentiated cells, were collected and analyzed as described in Material and Methods section. FC>2 corresponds to, at least, two-fold up-regulation of the gene, FC<2 corresponds to, at least, two-fold down-regulation of the gene in differentiated cells with RA. Data correspond to four separate experiments and differences are also represented in Fig 2. Abbreviation: Dif–genes related with differentiation process to a cardiac phenotype; CellSig–genes encoding proteins related with cell signaling pathways; Metabol—genes related with metabolic activity; Cellcycle—genes related with proliferation activity; Calcium–genes related with cellular calcium handling. Most relevant differences in terms of gene expression in H9c2 undifferentiated myoblasts vs RA-differentiated H9c2 cells.