Bacterial plasmids are self-replicating, extrachromosomal elements associated with a variety of phenotypic traits including virulence, enhanced fitness, and resistance to antimicrobial agents. They pl
Standard bacteriological media MHB (Sigma-Aldrich) was supplemented with 25 mg/L Ca2+ and 12.5 mg/L Mg2+ (CA-MHB). Eukaryotic cell culture media Roswell Park Memorial Institute 1640 (RPMI) (Thermo Fis