Daylight regulation of cardiac gene expression in wild type and Per2 deficient mice

Daylight is fundamental to human health and protects from myocardial ischemia in a PER2 dependent manner. Using a whole genome array from daylight exposed wildtype or Per2-/- mice, we investigated light elicited pathways.Mice were exposed to intense light (10,000 LUX, Lightbox simulating day light, Uplift Technologies DL930 Day-Light 10,000 Lux SAD, full spectrum) for 7 days and compared to mice maintained at room light [200 LUX]. Mice were housed in a 14/10-h light-dark cycle to synchronize (entrain) the circadian clock of mice to the ambient light-dark cycle.日光对人类健康至关重要，且可通过Period昼夜节律调节蛋白（PER2）依赖的途径发挥抗心肌缺血作用。本研究利用经日光暴露的野生型（wildtype）小鼠及Per2基因敲除（Per2-/-）小鼠的全基因组芯片（whole genome array），探究光诱导的信号通路。将小鼠分为两组：一组置于强光环境（10000勒克斯，模拟日光的光照箱：Uplift Technologies DL930全光谱日光10000勒克斯季节性情感障碍（SAD）治疗光照箱）中暴露7天，另一组则维持于200勒克斯的常规光照环境中作为对照。所有实验小鼠均饲养于14小时光照/10小时黑暗的光周期环境中，以使其生物钟与外界光周期同步（即授时，entrain）。