Gene expression profiling of alveolar macrophages from normal lung and metastasis-associated macrophages

To profile the gene expression of macrophages associated with lung metastases and identify mechanisms by which macrophages regulate metasetatic growth Lung metastases were induced by tail vein injection of syngenic mouse mammary tumor cells, dissected and processed in parallel with normal lungs for FACS sorting for the CD11B+ and CD11C+ subsets of CD45+;F4/80+;LY6C-;MRC1+;FSC hi cells. RNA from these cells was analyzed by RNA-seq.