Figure 1C_Heat Map of gH/gL binding screen

Mice were repeatedly immunized with HCMV purified virus or plasmid encoding gH/gL and blood was collected to evaluate the neutralization capacity of mouse sera in epithelial (ARPE-19) and fibroblast (MRC5) cells using reporter virus AD169R (MOI 0.1). Percent infection was quantified using GFP and normalized to virus incubated in the presence of normal mouse serum (NMS). Each serum condition was tested in technical triplicate (n=3). (c) Antibody clones capable of binding gH/gL were identified following hybridoma supernatant incubation with 293ExpiF cells transiently expressing gH/gL. Mean fluorescence intensity (MFI) was determined for each sample and summarized by fusion (n=1).