Next generation sequencing of circular RNAs in diabetes mellitus rats after sleeve gastrectomy

Sleeve gastrectomy (SG) can improve diabetes mellitus dramatically. However, the mechanisms remain largely undetermined. With the advancement of in-depth bioinformatic analysis, non-coding RNAs especially circular RNAs(circRNAs), have been implicated in many biological processes. To explore whether circRNAs mediate the amelioration of diabetes mellitus after SG, we subjected liver samples of diabetic rats after SG and shame operations for RNA sequencing. Through RNA sequencing, we identified a dramatically differentially expressed profile of 107 circRNAs. Some of these specific circRNAs were only expressed in diabetic rats after SG, which may be promising biological markers of SG prognosis.袖状胃切除术（Sleeve gastrectomy, SG）可显著改善糖尿病，但其具体分子机制仍未完全阐明。随着深度生物信息学分析技术的不断发展，非编码RNA（non-coding RNAs），尤其是环状RNA（circular RNAs, circRNAs），已被证实参与诸多生理及病理生物学过程。为探究环状RNA是否介导袖状胃切除术后糖尿病的改善效应，本研究对接受袖状胃切除术及假手术的糖尿病大鼠的肝脏样本开展RNA测序（RNA sequencing）。经RNA测序分析，本研究鉴定得到107个差异表达显著的环状RNA。其中部分特异性环状RNA仅在接受袖状胃切除术的糖尿病大鼠中表达，有望成为袖状胃切除术预后评估的潜在生物标志物。