The effect on the transcriptome by inhibition of mitochondrial function by metformin in intestinal cells. The effect on the transcriptome by inhibition of mitochondrial function by metformin in intestinal cells

We purified mouse duodenal cells from organoids derived from two different transgenic mouse lines (GIP-Cre tdRFP, SST-Cre tdRFP), and identified transcripts enriched in metformin-treated cells. Overall design: Comparative transcriptomic study of 4x cell populations by bulk RNAseq, with 3x biological replicates after vehicle control, and 3x biological replicates after 1mM metformin treatment.