Analysis of Fusarium verticillioides gene expression in response to nitric oxide (NO)

Investigation of whole transcriptional changes in F. verticillioides wild-type (WT) strain FRC M-3125 and gene deletion strain ΔFVEG_10494-6.4 when treated with and without 1.5 mM diethylenetriamine (DETA) NONOate for 30 min, with three biological replicates each. DETA NONOate is a NO-donor molecule. Overall design: Strains were grown for three days in potato dextrose broth (PDB) buffered with 0.1 M sodium phosphate. Prior to harvesting samples, the NO treatments received a 30 min exposure to 1.5 mM DETA NONOate dissolved in 0.01 M NaOH.

对弗氏萎黄菌野生型菌株FRC M-3125及其基因敲除菌株ΔFVEG_10494-6.4在1.5 mM二乙烯三胺（DETA）NONOate处理与否的条件下，经30分钟处理后的全转录组变化进行了研究。DETA NONOate作为一种NO供体分子。总体设计如下：菌株在含0.1 M磷酸钠的土豆葡萄糖 broth（PDB）中培养三天。在采集样本之前，接受NO处理的菌株需暴露于1.5 mM DETA NONOate溶液中，该溶液溶解于0.01 M氢氧化钠，持续30分钟。实验设置包括三个生物学重复。