small RNA sequencing of plasma miRNA from sepsis patients

Purpose: The aim of this study is to compare the plasma miRNA profile between healthy control and sepsis patients Methods: Plasma from healthy control and sepsis patients were used in the study. Total RNA was isolated from equal volume of plasma using Trizol LS. NGS cDNA libraries were prepared using Norgen Biotek Small RNA Library Prep Kit. Library quality was validated prior to sequencing on an Illumina NextSeq 500 platform. Plasma from eight healthy control and ten sepsis patients were used in this study. cDNA librarues were sequenced using Illumina NextSeq 500 platform.