Quantification of the translatome in wild type Arabidopsis and amiNAA10 plants

Wild type Arabidopsis Col-0 plants and mutants depleted of the catalytic subunit of the ribosome-associated N-acetyltransferase A (NatA) complex (amiNAA10) were grown on soil for six weeks under short-day conditions (8h light, light intensity: 100 µE, 21 °C/18 °C temperature (day/night), 50 % humidity). The translatome of both genotypes after 2 hours of light (10 am) was determined by feeding of the trackable Met-analogue azidohomoalanine for 4 hours to leaf disks floated on ½ Hoagland medium containing 50 µM azidohomoalanine in the light.