Integrin a3ß1-dependent mRNA expression profile of hair bulge keratinocytes and their progeny during DMBA/TPA-mediated skin tumorigenesis

Integrin a3ß1 in hair bulge population affects the tumor formation following DMBA/TPA carcinogenesis protocol, however linage tracing showed that hair bulge cells do not largely contributre to tumor mass. To investigate whether a3ß1 might affect tumor promoting environment and to better understand its role in hair bulge population during skin tumorigenesis, we determined the expresison profile of hair bulge-originating keratinocytes isolted from a3ß1KO and WT mice during initiation of tumorigenesis. Overall design: CRE-induced GFP-positive keratinoctes from K19(Cre-ERT); mTmG; Itga3 fl/fl and K19(Cre-ERT); mTmG mice, submitted to short-term DMBA/TPA treatment, were isolated using FACS and their expression profile was determined using next generation RNA sequencing