Modulating heterologous pathways and optimizing fermentation conditions for biosynthesis of kaempferol and astragalin from naringenin in Escherichia coli
收藏国家林业和草原科学数据中心2022-11-03 更新2024-03-06 收录
下载链接：
https://www.forestdata.cn/dataDetail.html?id=CSTR:17575.11.0220221103123.040001.V1
下载链接
链接失效反馈官方服务：
资源简介：
In this study, we constructed efficient biosynthesis pathways for kaempferol and astragalin production using combinatorial strategy, designing synthetic fusion enzymes and increasing gene copy number. By optimizing conversion and fed-batch fermentation conditions, we achieved maximal kaempferol production in BL21-F69-CisF3H at 1184.2 ± 16.5 mg/L, and maximal astragalin production in BL21-AST-II at 1738.5 ± 24.8 mg/L, representing the highest yields of kaempferol or astragalin from naringenin reported to date. These results indicated that this synthesis pathway represents a good candidate for flavonoid biosynthesis in E. coli.
提供机构：
国家林业和草原科学数据中心
创建时间：
2022-11-03
