Single cell sequencing of GFP+ Tri-PyMT cells from lung metastasis

Using an EMT lineage-tracing model, we identified tumor cells in the transitioning phases of both EMT and MET. Further analyses revealed that the ribosome biogenesis (RiBi) pathway was specifically elevated in these transitioning cells, regardless of their destined phenotypical fates. Overall design: GFP+ Tri-PyMT cells were sorted from parental culture of Tri-PyMT cells by flow cytometry and injected into SCID mice to let lung metastasis outgrowth. 15 days after injection, tumor bearing lungs of 3 mice were harvested and prepare for single cell suspensions. GFP+ cells were sorted for single cell RNA sequencing.