Loss of EIF4G2 Mediates Aggressiveness in Distinct Endometrial Cancer Subpopulations with Adverse Outcomes in Patients. Loss of EIF4G2 Mediates Aggressiveness in Distinct Endometrial Cancer Subpopulations with Adverse Outcomes in Patients

Low EIF4G2 protein expression was shown to have a poor OS of endometrial patients. Control and EIF4G2KD HEC-1A cells were sorted using CD133 merker to CD133+ and CD133- populations. These populations were taken to RNAseq and MS analysis to explore the mechanism. Overall design: HEC-1A endomertial cell line was KD with shRNA for EIF4G2 (DAP5) or shGFP (shNT) as control. The control and EIF4G2KD cells were subjected to sorting according to CD133 into CD133+ (p) and CD133- populations. The four populations were sorted as N=4 in independent experiments and save in the appropriate buffer according to the manufacturers protocol (Dynabeads™ mRNA DIRECT™ Purification Kit (Invitrogen)). mRNA was isolated and subjected to MARSseq2.0 protocol.