Pyruvate–GPR31 axis induces LysoDC dendrite protrusion to M-cell pockets for effective immune responses; Supplementary videos

Name: Pyruvate–GPR31 axis induces LysoDC dendrite protrusion to M-cell pockets for effective immune responses; Supplementary videos
Creator: figshare
Published: 2025-04-29T05:22:17+08:00

DataCite Commons2025-04-29 更新2025-05-07 收录

免疫生物学

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https://figshare.com/articles/dataset/_b_Pyruvate_GPR31_axis_b_b_induces_LysoDC_dendrite_protrusion_to_M-cell_pockets_for_effective_immune_responses_Supplementary_videos_b_/28888943 数据链接链接失效反馈

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Supplementary Video 1. Two-photon microscopic imaging of a Peyer's patch (PP) explant prepared from untreated Gpr31b+/+ Cx3cr1gfp/+ mice. PP explants prepared from untreated Gpr31b+/+ Cx3cr1gfp/+ mice were stained with anti-GP2 antibody (red) and vertically monitored with a z-step interval of 0.5 µm by two-photon microscopy. Arrows indicate LysoDC dendrites in M-cell pockets. The arrows remain in place after the image has moved to a depth where the dendrites are not visible. Scale bar, 50 µm. Video corresponds to Figure 2(a).Supplementary Video 2. Two-photon microscopic imaging of a PP explant prepared from untreated Gpr31b-/- Cx3cr1gfp/+ mice. PP explants prepared from untreated Gpr31b-/- Cx3cr1gfp/+ mice were stained with anti-GP2 antibody (red) and vertically monitored with a z-step interval of 0.5 µm by two-photon microscopy. Arrows indicate LysoDC dendrites in M-cell pockets. The arrows remain in place after the image has moved to a depth where the dendrites are not visible. Scale bar, 50 µm. Video corresponds to Figure 2(a).Supplementary Video 3. Two-photon microscopic imaging of a PP explant prepared from untreated Gpr31b+/+ Cx3cr1gfp/gfp mice. PP explants prepared from untreated Gpr31b+/+ Cx3cr1gfp/gfp mice were stained with anti-GP2 antibody (red) and vertically monitored with a z-step interval of 0.5 µm by two-photon microscopy. Arrows indicate LysoDC dendrites in M-cell pockets. The arrows remain in place after the image has moved to a depth where the dendrites are not visible. Scale bar, 50 µm. Video corresponds to Figure 2(a).Supplementary Video 4. Two-photon microscopic imaging of a PP explant prepared from pyruvate-treated Gpr31b+/+ Cx3cr1gfp/+ mice. PP explants prepared from pyruvate-treated Gpr31b+/+ Cx3cr1gfp/+ mice were stained with anti-GP2 antibody (red) and vertically monitored with a z-step interval of 0.5 µm by two-photon microscopy. Arrows indicate LysoDC dendrites in M-cell pockets. The arrows remain in place after the image has moved to a depth where the dendrites are not visible. Scale bar, 50 µm. Video corresponds to Figure 2(a).Supplementary Video 5. Two-photon microscopic imaging of a PP explant prepared from pyruvate-treated Gpr31b-/- Cx3cr1gfp/+ mice. PP explants prepared from pyruvate-treated Gpr31b-/- Cx3cr1gfp/+ mice were stained with anti-GP2 antibody (red) and vertically monitored with a z-step interval of 0.5 µm by two-photon microscopy. Arrows indicate LysoDC dendrites in M-cell pockets. The arrows remain in place after the image has moved to a depth where the dendrites are not visible. Scale bar, 50 µm. Video corresponds to Figure 2(a).Supplementary Video 6. Finger-like dendrite of LysoDCs in M-cell pockets. PP explants prepared from untreated Gpr31b+/+ Cx3cr1gfp/+ mice were monitored as described in Supplementary video 1. Volume rendering-based 3D reconstruction of the LysoDC protruding a finger-like dendrite into an M-cell pocket is shown. Video corresponds to Figure 2(e).Supplementary Video 7. Spherical dendrite of LysoDCs in M-cell pockets. PP explants prepared from pyruvate-treated Gpr31b+/+ Cx3cr1gfp/+ mice were monitored as described in Supplementary video 4. Volume rendering-based 3D reconstruction of the LysoDC protruding a spherical dendrite into an M-cell pocket is shown. Video corresponds to Cell #2 in Figure 2(g).

补充视频1. 未处理的Gpr31b+/+Cx3cr1gfp/+小鼠派尔集合淋巴结（Peyer's Patch, PP）外植体的双光子显微镜成像 本实验对未处理的Gpr31b+/+Cx3cr1gfp/+小鼠的PP外植体采用抗GP2抗体（红色）进行染色，并通过双光子显微镜以0.5 μm的z轴步长间隔进行垂直层面扫描成像。箭头指示微皱褶细胞（M细胞）囊腔内的溶酶体树突状细胞（LysoDC）树突；当图像切换至树突不可见的深度层面时，箭头位置保持不变。比例尺为50 μm。本视频对应图2(a)。 补充视频2. 未处理的Gpr31b-/-Cx3cr1gfp/+小鼠派尔集合淋巴结外植体的双光子显微镜成像 本实验对未处理的Gpr31b-/-Cx3cr1gfp/+小鼠的PP外植体采用抗GP2抗体（红色）进行染色，并通过双光子显微镜以0.5 μm的z轴步长间隔进行垂直层面扫描成像。箭头指示M细胞囊腔内的LysoDC树突；当图像切换至树突不可见的深度层面时，箭头位置保持不变。比例尺为50 μm。本视频对应图2(a)。 补充视频3. 未处理的Gpr31b+/+Cx3cr1gfp/gfp小鼠派尔集合淋巴结外植体的双光子显微镜成像 本实验对未处理的Gpr31b+/+Cx3cr1gfp/gfp小鼠的PP外植体采用抗GP2抗体（红色）进行染色，并通过双光子显微镜以0.5 μm的z轴步长间隔进行垂直层面扫描成像。箭头指示M细胞囊腔内的LysoDC树突；当图像切换至树突不可见的深度层面时，箭头位置保持不变。比例尺为50 μm。本视频对应图2(a)。 补充视频4. 丙酮酸处理的Gpr31b+/+Cx3cr1gfp/+小鼠派尔集合淋巴结外植体的双光子显微镜成像 本实验对丙酮酸处理的Gpr31b+/+Cx3cr1gfp/+小鼠的PP外植体采用抗GP2抗体（红色）进行染色，并通过双光子显微镜以0.5 μm的z轴步长间隔进行垂直层面扫描成像。箭头指示M细胞囊腔内的LysoDC树突；当图像切换至树突不可见的深度层面时，箭头位置保持不变。比例尺为50 μm。本视频对应图2(a)。 补充视频5. 丙酮酸处理的Gpr31b-/-Cx3cr1gfp/+小鼠派尔集合淋巴结外植体的双光子显微镜成像 本实验对丙酮酸处理的Gpr31b-/-Cx3cr1gfp/+小鼠的PP外植体采用抗GP2抗体（红色）进行染色，并通过双光子显微镜以0.5 μm的z轴步长间隔进行垂直层面扫描成像。箭头指示M细胞囊腔内的LysoDC树突；当图像切换至树突不可见的深度层面时，箭头位置保持不变。比例尺为50 μm。本视频对应图2(a)。 补充视频6. M细胞囊腔内LysoDC的指状树突 本实验对未处理的Gpr31b+/+Cx3cr1gfp/+小鼠的PP外植体按照补充视频1中的方法进行成像观测，展示了伸入M细胞囊腔的LysoDC指状树突的基于体绘制（volume rendering）三维重建结果。本视频对应图2(e)。 补充视频7. M细胞囊腔内LysoDC的球状树突 本实验对丙酮酸处理的Gpr31b+/+Cx3cr1gfp/+小鼠的PP外植体按照补充视频4中的方法进行成像观测，展示了伸入M细胞囊腔的LysoDC球状树突的基于体绘制（volume rendering）三维重建结果。本视频对应图2(g)中的细胞#2。

提供机构：

figshare

创建时间：

2025-04-29

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数据集介绍