Not just flowering time: a resurrection approach shows floral attraction traits are changing over time

Contemporary anthropogenic changes in climate and landscape form a complex set of selective pressures acting on natural systems, yet, in many systems, we lack information about both whether and how organisms may adapt to these changes. In plants, research has focused on climate-induced changes in phenology and the resultant potential for disruption of plant-pollinator interactions, however, there remains a paucity of knowledge regarding how other pollinator-mediated traits may be involved in the adaptive response. Here, we use resurrection experiments to investigate the phenotypic basis of adaptation in a mixed-mating system plant, the common morning glory (Ipomoea purpurea). Specifically, we measure temporal and spatial changes in traits grouped into three categories relevant to plant-pollinator interactions - floral morphology, floral rewards, and floral phenology. We show a significant temporal increase in corolla size and shift to earlier flowering times, as well as a potential for ..., This dataset includes a set of phenotypes measured directly from live plants growing in greenhouse conditions. There is information both about the natural populations from which seeds were collected (population name, year of collection, and latitude/longitude) as well as measurements of floral morphology (corolla length, corolla width, and anther-stigma distance), phenology (date of first flower), and floral rewards (sugar content in nectar and pollen counts) done in three separate resurrection experiments., Floral Morphology
This data file contains floral morphology data for 15 populations distributed in the SE United States and sampled in both 2012 and 2003. Latitude and longitude for each population are included as columns.
floralMorphology.csv
 
Phenology

This data file contains date of first flower data for 23 populations distributed in the SE United States and sampled in both 2012 and 2003. Latitude and longitude for each population are included as columns.
phenology.csv
 
Floral Rewards
There are two data files, one of which contains Brix scale measurements of sugar content in nectar and the other of which contains pollen counts. Both sets of data are taken from 4 populations in the SE United States sampled in both 2012 and 2003. There is missing data for flower color in the Brix dataset - all missing data is marked with \"na\". 
nectarBrix.csv, pollenCount.csv
 
R Code
R Code in markdown format plus two additional external scripts that are necessary for running t..., # Not just flowering time: A resurrection approach shows that floral attraction traits are changing over time

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Four .csv data files

#### 1. floralMorphology

This dataset includes floral measurements from ~6 flowers/plant in a resurrection experiment done with Ipomoea purpurea (the common morning glory).
Seeds were collected from 15 populations in the SE United States at two time points, 2003 and 2012, and grown together in a common garden greenhouse experiment.
Floral measurements included are corolla width, corolla length, and the distance between the tallest anther and stigma.
Results show directional selection for increased corolla width over time, no change in corolla length over time, and balancing selection on anther-stigma distance.
Other information in the datasheet includes a population ID number, name, and location (latitude and longitude) for each population. Further results indicate differential selection across latitudes.

#### 2. phenology

This dataset includes phe..., , **Changes after Nov 18, 2024:** Re-uploaded floralMorphology.csv, pollenCount.csv, and phenology.csv because they were inaccessible to public users. No changes were made to the datasets.