Detailing Novel Epigenetic Events Associated with Diet-Induced Obesity in the Rat

We applied Whole Transcriptome Termini Site Sequencing (WTTS-seq) to detect differences in use of alternative polyadenylation sites between diet-induced obesity rats and control rats. Our finding clear showed that a nutritionally complete high fat diet would affect pre-translational alternative polyadenylation events on hypothalamic transcripts that both stimulate and counter regulate body weight.本研究采用全转录组末端位点测序（Whole Transcriptome Termini Site Sequencing, WTTS-seq）技术，检测饮食诱导肥胖大鼠与对照大鼠在可变多聚腺苷酸化位点（alternative polyadenylation sites）使用模式上的差异。研究结果明确显示，营养完全的高脂饮食会对下丘脑转录本上的翻译前可变多聚腺苷酸化事件产生影响，此类事件可双向调控机体体重。