IL-25-induced effector-memory ILC2s mediate small intestinal adaptation [ATAC-seq]

This study used bulk ATAC sequencing of either small intestinal ILC2s or Lgr5+ epithelial stem cells +50 days after an IL-25 regimen or PBS regimen in wild type (ILC2s) or Lgr5DTR-EGFP (epithelial stem cells) mice to study chromatin accessibility. Overall design: Mice (wild type and Lgr5DTR-EGFP) were injected i.p. with IL-25 (500ng) or PBS 3x a week for 4 weeks and then rested for 50+ days. ILC2s (KLRG1+, CD3-, CD8a-, CD4-, CD19-, NKp46-, NK1.1-, F4/80-, Gr1-, CD11b-, CD11c-, Ter119- ) were then sort purified from the small intestine lamina propria. Lgr5+ intestinal stem cells (Lgr5GFP+, CD44+, EpCAM+, CD45-) were then sort purfied from the small intestine epithelium. Cells were then used for bulk ATAC sequencing.