Benchmark of existing methods for predicting coiled-coil specificity.

Experimentally determined Kd values were compared to predicted scores using two tests: the Pearson correlation coefficient (R) and the area under the curve (AUC). The Pearson correlation coefficient is reported only for interactions with 1 nM d d d ≥ 5,000 nM). The number of interactions used in each test is given in parentheses at the top of each column.a Vinson and colleagues measured coupling energies for pairs of residues in ai-a'i and gi-e'i+1 positions. An additional empirical coupling energy of -2 kcal/mol for Leu-Leu interaction at di-d'i positions was added to account for these strongly stabilizing interactions, as in [16].Benchmark of existing methods for predicting coiled-coil specificity.