TGF-Ã specifies TFH versus TH17 cell fates in murine CD4+ T cells through c-Maf (Seq03, bulk-RNAseq)
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https://www.ncbi.nlm.nih.gov/sra/SRP361392
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Different numbers of naive murine CD4+ T cells were cultured at different seeding cell densities and under different polarizing conditions in 96-well plates coated with anti-CD3/anti-CD28 for 3.5 days in the presence of 2 or 5 ng/ml TGF-Ã (Tfh2 or Tfh5) or with anti-TGF-Ã (TfhA), sorted by flow cytometry, and subjected to bulk RNA-seq. Overall design: Seq03: bulk RNA-seq: Tfh(2)1e4, Tfh(2)2e4, Tfh(2)4e4, Tfh(2)6e4, Tfh(5)4e4, Tfh(A)4e4
创建时间:
2024-03-05



