Data from: A long PCR based approach for DNA enrichment prior to next-generation sequencing for systematic studies

Premise of the study: We present an alternative approach for molecular
systematic studies that combines long PCR and next-generation sequencing
(NGS). Our approach can be used to generate templates from any DNA source
for NGS. Here we test our approach by amplifying complete chloroplast
genomes and we present a set of 58 potentially universal primers for
angiosperms to do so. Additionally, this approach is likely to be
particularly useful for nuclear regions. Methods and Results: Chloroplast
genomes of 30 species across angiosperms were amplified to test our
approach. Amplification success varied depending on whether PCR conditions
were optimized for a given taxon. To further test our approach, some
amplicons were sequenced on an Illumina HiSeq 2000. Conclusions: Although
here we tested this approach by sequencing plastomes, long PCR amplicons
could be generated using DNA from any genome, expanding the possibilities
of this approach for molecular systematic studies.