Effect of rhpn2 knowout on gene expression of zebrafish embryos at 72 hpf (hours post fertilization) .

Hearing impairment due to hair cell damage has always been a problem for deaf patients，and there are many unknown genes for deafness.Presently, we have established a zebrafish model that exhibits significant hearing loss induced by hair cell damged with rhpn2 was knockouted. Utilizing this model, we analyzed and compared the differentially expressed genes of wild type and rhpn2 mutant embryos. The results revealed that 631 genes were down-regulated significantly in rhpn2 mutant embryos, respectively, compared with control. Wild type and rhpn2 mutants embryos were collected for transcriptome sequencing at 72 hpf .